Factors that can influence the growth rate of HFLS cells include:
Passage number: Higher passage number cells may exhibit slower growth rates compared to early passage cells. It is important to monitor the passage number and ensure cells are used within the recommended range.
Seeding density: The initial cell seeding density can impact the time required for cells to reach confluency. Adjusting the seeding density can help optimize the growth rate and achieve the desired confluency within a reasonable time frame.
Growth medium: The composition of the growth medium, including the presence of growth factors and supplements, can affect cell proliferation rates. Ensuring the use of appropriate growth medium and supplements specific for HFLS cells can help optimize their growth.
Culture conditions: Factors such as temperature, humidity, and CO2 levels in the incubator can influence cell growth rates. Maintaining optimal culture conditions, as recommended for HFLS cells, can support their growth and proliferation.
The HFLS cells grow slowly and have a doubling time of 5–7 days. Because of cell senescence, use of fibroblast-like synoviocytes after passage 9 is generally not recommended. The proliferation gradually starts to decline after passage 9.
Please refer to the article attached below for more information.
Article Acquisition, Culture, and Phenotyping of Synovial Fibroblasts