Simultaneous imaging of multiple components (Carbohydrates, Fat, Protein) in food products using confocal microscopy??

Hi Everyone, I am trying to characterize the microstructure (localization of carbohydrates, fats, and proteins) of coconut milk powder using confocal microscopy. From the review of the available...

12 January 2021 5,464 2 View

Is it possible to separate Supleco 37 Fame mix using HP-5MS column?

Hi everyone, I am new to FAME analysis. I am trying to separate Supleco 37 Fame mix using the HP-5MS (30 m * 0.25 mm ID * 0.2 um thickness) column using the following protocol: System: Agilent...

19 December 2020 7,356 4 View

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30 November 2020 5,089 5 View

Transcription mediated amplification (TMA)?

I am trying to optimize TMA in my lab. When I start with 150 pg of DNA (Size 235 bp), I get good amplification, which I can visualize as an equivalent size dsDNA product, gel pic attached....

18 November 2020 7,622 3 View

How to do data analysis of a split plot design experiment with an extra control treatment outside the design but in the same field?

In an split plot experiment, 4 methods of Nitrogen(N) application was assigned in main plot and 3 doses of N in subplots and replicated thrice. Along with it, in the same field, No N control...

20 September 2020 9,944 2 View

How to use WRF output data as input data to WRF for initiating new simulation for higher resolution domain?

I want to run WRF-Real at higher resolution (100 m). For that I have to downscale WRF output data (1 Km).

13 July 2020 1,948 2 View

How do we do intersection of two raster image in ENVI?

After threshold the value of Moran Geary and Getis index using Local statistics in Envi how we get the common part between Moran and Geary indices

05 April 2020 6,234 3 View

How to generate large scale forcings like subsidence, advective tendencies and radiative cooling rate; from reanalysis data for Large eddy simulation?

For performing LES initial soundings have been taken from ERA data. But large scale forcings and surface fluxes also needs to be computed, to provide these as input. Kindly suggest some suitable...

22 October 2019 6,165 4 View

Can I use organisational commitment in my paper when I am only using affective commitment and normative commitment?

In my paper, I am only testing the effects of affective commitment and normative commitment but not continuous commitment. I keep having a problem in my paper when I am creating a hypothesis. Can...

18 September 2019 3,942 1 View

PLS HELP! What is the best SPSS test for this research study?

Hello I have completed a study examining how training effects employees intention to remain in the organisation. Below are the relevant variables and information needed: Question 1. What is the...

09 September 2019 306 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Which is better medicine or food in obesity?

Preference in terms of the scientific aspect

28 February 2021 796 2 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

21 February 2021 374 5 View

How to perform EEG classification of Alzheimer and control groups?

I am doing a project on classification of EEG signals of Alzheimer's disease and control groups. Previously, I extracted the alpha power of the EEG signals and found out the strengths. I want a...

18 February 2021 8,502 2 View

Can ImageJ software be used to quantify collagen from Masson's-Trichrome stained images?

I am after quantifying the blue stained collagen in a Masson's-Trichrome stained skin section from an immune deficient mice and compared the same with its WT control. I am curious to know can...

17 February 2021 384 3 View

Is there a way to grow biofilm in lab to mimic biofilms in showers and baths? Also, how can I measure it?

Hello, I am new to biofilm research and have a question about growing and measuring biofilms. We want to research the effect of a type of enzymes in breaking down biofilms in showers, baths, hot...

14 February 2021 3,522 3 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View