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19 January 2021 7,478 3 View

Multiple sequence alignment - polymorphism for restriction digest?

Hi, After comparing multiple (2+) DNA sequences, I want to identify ONLY the restriction sites that changed due to the detected polymorphism. I tried several things by hand, but it's WAY too...

23 September 2020 6,289 4 View

Would you be interested in the special issue "High Temperature Statistical Thermodynamics of Molecules in Gases and Plasmas", Entropy IF=2.494?

https://www.mdpi.com/journal/entropy/special_issues/Gases_and_Plasmas

26 August 2020 9,474 4 View

Can I run a chi-square test when my data include one 'zero' value? Can I run a post hoc analysis after chi-squre when p<0.05?

I have one row of raw data 13, 0, 24, 26, 17 and 14. It is a larval survival rate / six treatments. Can I run chi-squared with zero inluded? I use R, and it looks like it works, but I am not...

24 July 2020 7,720 5 View

How to identify crystals in the shrink cavity?

I have a problem. I watched the crystals on the surface of the shrinking cavity. How to recognize these elements? EDS analysis is not possible due to the depth of the shrink cavity. Machining can...

29 May 2020 4,235 0 View

How to choose the right protein subunit for symmetrical docking?

Recently, I have been dealing with symmetrical docking of protein complexes and I have a problem. Well, I have no problems with docking complexes with cyclic symmetry, it is double-walled symmetry...

09 May 2020 4,424 3 View

How to model a material in Abaqus with 2 types of behavior: high stiffness in compression and low stiffness in tension?

I am modeling a dynamic problem in Abaqus. The material should have two types of properties: different Young's moduli in tension and compression. Any simple way to achieve it? Thanks!

17 February 2020 8,563 2 View

How many water molecules are incorporated into the structure of fructan?

I am looking for help to calculate how many water molecules are incorporated into the structure of fructan. We want to apply polymer density functional theory (DFT) for prediction and description...

01 April 2019 7,096 3 View

No title

I test preference and survival rate of one population (moth larvae). I have six varieties of plants (a-f) in six replicates (36 in total) arranged in Latin square block. I applied 5 larvae (1st...

04 March 2019 5,183 3 View

How to understand "no change in luminescence band" when I can see this change?

26 June 2017 5,786 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View