Can we say that the flux of a protein (no of molecules present at a particular time in unit area) is equal to the expression of the protein in that tissue?
by flux you mean activity of the protein? (that the protein converts in particular tissue/cell lets say 5 molecules per second?)
If so, not really. Because there are also post-translational modifications, which affect the activity, so the protein may be expressed but due to PTM it may not be active.
Technically Flux of a protein should not be equivalent to its expression....As we might be knowing that protein present at instant may depend on its expression plus its stability factors....i.e some time expression of protein is constant but at particular condition...the protein stability is increased or decreased through regulation of various proteosomal mediated degradation machinery....
Dear Ajithavalli, protein flux and protein expression, in some conditions may be equal. But they are not the same necessary.
If expression increase and protein destruction increase too, the flux is not change but as state at first, expression increased.
So, without changing protein clearing process, flux can be equal as expression.
best
mehdi
The definition of flux is different from physics or maths where flux is treated as rate of flow of a property per unit area.
Flux of a metabolite is expressed as rate of production/consumption/transportation per gram Dry wt. of cell, wrt a particular bio-chemical reaction. Eg: Max glucose uptake rate for a exponentially growing E. coli, to a physiologically realistic level, is 18.5 mmol glucose gDW−1 h−1; DW, dry weight.
This is a good paper to understand FBA using COBRA toolbox of MATLAB http://www.nature.com/nbt/journal/v28/n3/full/nbt.1614.html
Cheers,
Shreyans
- Badges
- Science method
- Similar topics
- Biological Science
- Genetics
- Gene Expression
- Protein Expression