What are the factors responsible for non uniform decrease in corrosion rate?

I am working on the corrosive effect of food condiments juices on some selected metals using weight loss experiment. But while plotting the graph, I found out that the decrease in corrosion rate...

21 January 2021 6,688 4 View

What statistical technique is the most appropriate?

Hello! can anyone help? I'm currently working on a research paper, where I created a questionnaire containing 15 items related to challenges imposed by climate change. Respondents are supposed to...

18 March 2020 2,402 4 View

Why is Patch Dock returning my docking search?

the Patch Dock is giving me trouble regarding the code for my ligand the error message is " Error in receptor molecule input: please specify PDB code or upload file " My ligand is microRNA. and it...

19 June 2019 2,638 2 View

What database can i find the expressions of colorectum as a tissue ?

Dear all, I want to extract the genes related to colorectum but the database TIGER only showed the tissue view of colon. please suggest any database that can give the ESTs or expression profile of...

29 April 2018 5,910 4 View

Which online target prediction tool is best for predicting miRNA targets considering a friendly interface ?

I need to identify some targets of a number of miRNAs but i need suggestions on the best target prediction tool or a combination majorly for humans. Thanks

29 April 2018 1,323 2 View

How can I obtain Marginal effect on SPSS Tobit regression?

Hello readers, I would like to know how to obtain the marginal effect of my tobit regression on SPSS. I prefer SPSS to STATA simply because it give me the flexibility of dividing my variables into...

01 March 2018 6,044 3 View

CFD simulation with a constant wall temperature boundary condition error in abaqus?

26 September 2017 8,692 6 View

Is there any procedure to determine the difference in blood between normal and diebetic individual ?

22 January 2017 7,450 5 View

What dictates the specific target of microRNA for biomarker and therapeutics ?

One miRNA could have several targets, but these targets may not all be regulated by the miRNA in every circumstance. For standard utilization of miRNAs as biological function and to prevent the...

20 April 2016 1,547 2 View

Good morning, Please, how do i preserve the gastrointestinal tract of fishes for E.L.I.S.A viral analysis?

Please, i how do i preserve the gastrointestinal tract of fishes for E.L.I.S.A viral analysis?

19 April 2016 8,249 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

Alternatives to .45 um filter to isolate MS2 from lysed E. coli?

Hello, I am looking for alternatives or cheaper materials to isolate out MS2 from E. coli following lysis via chloroform. Some literature I have reviewed uses a .45 um nanopore filter following...

02 February 2021 807 3 View

What are some good protocols for native outer membrane protein extraction from E. coli?

I am trying to extract any and all outer membrane proteins from a lab strain of e. coli (ATCC 11229) and am looking for the best general protocol to extract outer membrane proteins. All...

25 January 2021 5,736 3 View

What are the main reasons to grow Escherichia coli O157:H7 and Salmonella Typhimurium in food products under refrigeration storage 4 C?

What are the main reasons to grow Escherichia coli O157:H7 and Salmonella Typhimurium in food products under refrigeration storage 4 C?

24 January 2021 9,987 3 View

Does increasing antibiotic concentration also increase plasmid production in DH5a E.coli?

Dear all, I am propagating some plasmids with a human gene and an ampicillin resistance gene. My logic is that if ampicillin concentration will be increased, the concentration of plasmid (pSF)...

14 January 2021 779 3 View

Recommendations for a microbial strain repository?

I'm looking for an open repository or culture collection to deposit some genetically engineered E. coli strains (from the papers below). The primary goal is to preserve those strains for the long...

11 January 2021 4,398 1 View

Why nitrocefin is preferred over CENTA?

The chromogenic beta-lactamase substrate CENTA is at least five times cheaper than the other famous substrate nitrocefin and can be used with most enzymes, still, nitrocefin is preferred. Any...

17 December 2020 3,735 2 View

Greiner Bio or Corning Costar plates for ELISA?

09 December 2020 237 2 View