How to regenerate PES Membrane of centrifugal filtration tubes having 5 and 50kDa MWCO?

Dear Collegues, I am using a relevant number of centrifugal filtration tubes made of polyethersulfone (PES) with 2 different MWCO, 5 and 50kDa for separation of protein in milk. They are quite...

15 December 2020 7,951 3 View

How to sterilise maize seeds in the lab?

What is the most effective protocol to sterilise maize seeds in the lab? We have tried few common procedures but with no luck (still get fungus contamination on agar plates): 1)10% sodium...

19 November 2020 602 15 View

How I can simulate solid state sintering?

I want to simulate solid state sintering of metal powder, conducted under vacuum. I am interested in the initial stage of sintering, does not matter to reach full density. Do you know any model or...

24 September 2020 7,383 5 View

Is there a good library to perform Petri net simulation in python?

Hello, I have designed several petri nets for my research. I am wondering how can I simulate them and gather system performance measure (eg sojourn times, throughput) using python? Is there a...

11 May 2020 2,120 2 View

What are the relevant economic, technical and environmental aspects of the recycling of batteries for automotive applicantions?

A paper review. Could you please provide some researches about the topic?

28 April 2020 946 5 View

Difference between photovoltaic plant short-circuit current and synchronus generator short-circuit current in AC transmission system?

Hello, I understand that photovoltaic plants are able to disconnect very quickly if there is a fault in the grid (cause power electronics), while synchronus generators cant disconnect due to his...

30 October 2019 8,457 5 View

Psychic salience has been studied in his correlation with psychosis.What about psychotropic drugs effect?

Some drugs seems to raise the feeling of psychic salience while others dont.

20 September 2018 3,556 1 View

In psychotic patients, psychotic features of the speech can vary their gravity during an interview. What do you think can be the influential factors?

In my view, these factors can be: the emotional meaning of the theme for the patient, anxious sympyoms caused by the situation or the interviewer, a relatedness feeling with the interviewer, a...

03 September 2018 303 3 View

Which assay to detect human serum levels of anti-troponin-I, anti-troponin-T and anti-tropomyosin antibodies?

We are currently using indirect ELISA to measure human serum levels of anti-troponin-I, anti-troponin-T and anti-tropomyosin antibodies? This indirect ELISA is not sensitive enough for our...

23 May 2018 6,546 2 View

Ksp in aprotic polar solvent , any idea?

11 May 2018 2,772 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

How to delaminate copper in water-based anodes?

Hello everyone, I'm looking for a way to delaminate copper current collector of water-based anodes in Li-ion batteries without damaging the graphite film. I tried different materials such as HNO3...

24 February 2021 3,785 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

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23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

QTL, recombination frection?

22 February 2021 2,588 2 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View