Hi all.
I am totally new to this type of experiment- 3D culture. I am planning to study MCF10A cell line in a 3D environment. I am following these two publications as the protocol- doi:10.1016/S1046-2023(03)00032-X and DOI:10.1038/NMETH1015. I have some queries related to the method-
1) Are matrigel and EHS tumor basement membrane same in components? Because I found that, Matrigel is the trade name for a gelatinous protein mixture secreted by Engelbreth-Holm-Swarm (EHS).
2) Is matrigel enough for acini formation with MCF10A cell line? Or, we need collagen in addition?
3) If I use cell culture dishes or multiplates for acini formation, how can I prepare the acini for immunostaining? Do we need to detach them form plate as ultimately we have to have a slide for confocal microscopy?
Thanks in advance. Your suggestions will be very much helpful for me.
Thank you Arman Javadi .
Another thing, do we really need slide chamber for acini formation or regular cell culture dishes/plates are good enough? Are there some extra benefits in using slide chambers?
I am not sure I just grew it in slide chambers. Slide chambers make it easier for handling spheroids and fixing them for immunofluorescence analysis.
I think you can use regular cell culture dishes as well.
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