Is cDNA obtained from random hexamer primer can be applied for sequencing (transcripomics)?

16 January 2024 1 4K Report

The protocol was designed for RNA sequencing can't be applied because RIN number was low and the cDNA for the same sample was obtained by random hexamer primer. What can i do to fix the situation because i can't do the process of RNA extraction again

Frederic Lepretre

Hi Mustafa

if it's for whole transcriptome, you can be interested in the 3' RNAseq which offers good results even with poor RIN RNA samples.

just read this paper:

Article A comparison between whole transcript and 3’ RNA sequencing ...

all the best

fred

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Science method

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