There is no need to do a cleanup before qPCR. Usually, you are diluting your cDNA quite a lot, so also the undesireed components of the RT reaction are diluted. In addition, a large fraction of the random primers used for RT will be consumed during the reaction.
There is no need to do a cleanup before qPCR. Usually, you are diluting your cDNA quite a lot, so also the undesireed components of the RT reaction are diluted. In addition, a large fraction of the random primers used for RT will be consumed during the reaction.
You can use RNA from the start to do you qPCR. It will be done in 2steps where the 1st will be creating cDNA for the genes you are targeting.
As far as i know, we only need to further cleanup the RNA not the cDNA. To determine quality and amount of cDNA you just need to test it with housekeeping gene across your samples (run normal PCR).
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