How should I dispose of silver staining waste (SDS-PAGE)?

I know I cannot pour silver staining waste down the sink, but how should I dispose of it? Thank you in advance!

18 November 2020 1,745 3 View

Do you know a not very expensive journal of Metagenomics?

Hi you all, I'm looking for a not very expensive journal to submit our work related to microbial diversity in a beverage drink using an amplicon perspective. Any suggestion is welcome because I...

02 May 2020 2,165 4 View

Can you help me with a lab protocol (RT-PCR, capillary electrophoresis, etc) to detect the fusion gene FIP1L1-PDGFRa in hypereosinophilia disorders?

I need a molecular biology protocol to detect this fusion gene FIP1L1-PDGFRa in hypereosinophilia disorders, because the protocol that I have is not working, and I see several inespecifical bands...

13 February 2020 1,304 4 View

Did anyone of you ever resuspended compounds such as beta sitosterol or campesterol in culture medium?

I noticed that these compunds are not soluble in DMSO, and DMSO damages hepatocarcinoma cells a lot. Thank you!

04 February 2020 6,575 1 View

How many mice do I need to do qPCR of T cells sorted from B16 TILs?

03 February 2020 9,215 2 View

Why is the TMB substrate turning blue without being in contact with the sample and in the dark?

Hi everyone, last time I was doing an ELISA without exposing it to the light and before I added the substrate it was turning blue. Do you have any idea or suggestion of a better substrate?, the...

03 January 2020 1,294 7 View

Why my Cell Cycle evaluation by Flow cytometry IS FAILLING?

Hello I'm trying to evalute the cell cycle in a lung cell line (NL-20) by flow cytometry I'm new in this type of experiments. I have used PI and DRAQ5 fluorocrhomes, but i only get 1 peak in my...

09 October 2019 5,029 4 View

How to prevent 68Ga-colloid formation?

Hi, I am currently trying to optimize the radiolabeling of a peptide (w/ NOTA) but I am ending up with 30% 68Ga-colloid in the reaction. I am using HEPES as the buffer and setting the reaction pH...

06 October 2019 1,181 2 View

How can i know if my cell type expresses a certain type of enzyme?

In an informatic database, like typing the name of the enzyme and then the information of the enzymes expressed in that particular cell type.

16 May 2019 2,893 3 View

Can you combine causal research design and exploratory research design?

its for business dissertation for masters and i wanted to know whether you could combine research designs

27 March 2019 4,706 6 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

21 February 2021 374 5 View

What should a smart water system look like?

Our team is designing a smart water C&C dashboard for a smart city initiative. As we look into the current smart water system landscape, we couldn't reconcile to a definitive solution that...

16 February 2021 1,094 3 View

Any recommendations for methods to analyze elephant space-use (using GPS) and correlating this with changes in NDVI?

For my Masters I am looking into elephant space-use and its effect on vegetation change. I have elephant GPS collar data and was going to use Google Earth Engine to obtain NDVI values. I would...

16 February 2021 7,097 3 View

How to consider effects of rapid warm adaptation in modeling of ecological impacts of warming?

I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...

15 February 2021 3,642 6 View

Extraction of DNA from insect samples conserved in silica gel?

Hi everybody, can anybody suggest how to extract DNA from insect samples conserved in silica gel (in my case, mosquitoes)? Does anybody know a commericial kit which does that or a particular...

10 February 2021 3,392 4 View

MAD or boxplot to detect outliers in non normally distributed data for latency and distance in animal cognition?

I am studying animal cognition and I have data about animals' latency to approach a cue and animals' distance from the cue in a test. Data are not normally distributed and when I do scatterplots...

04 February 2021 503 3 View

What is the magnitude of yearly rhizopdeposition in tropical forests?

Hi all, I am trying to find reference values on annual soil rhizodeposition rates for different kinds of forests, with a particular interest for tropical rainforests. Do we have an idea of the...

26 January 2021 4,278 4 View

Can you recommend a good book on analytical chemistry for non-chemists?

Dear colleagues, I am asking for you recommendation: I need a book on analytical chemistry in environmental sciences. As a non-chemist (a biologist) I have some basic chemistry knowledge but not...

25 January 2021 8,486 8 View