Is anyone in possession of pEC86 plasmid???

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How effective is the Citi Bloc standard basket in enhancing the accuracy and comparability of international construction cost assessments?

Citi BLOC Standard Basket Definitions: A standardized unit representing a fixed basket of construction materials, labor, and equipment costs priced in various cities. Purpose: To create a common...

04 August 2024 8,997 1 View

How long takes to a batholith to crystallize?

How long would it take for a 'normal' granitic batholith to crystallize? Are there any calculations on it?

12 July 2024 7,436 0 View

How will you write a literature review on impact of galamsey on agricultural land in Ghana?

A research proposal on impact of galamsey on agricultural land in wassa

12 July 2024 6,478 3 View

Why my comet have halo shape in comet assay ?

Hello every one, I am trying to perform comet assay. But my comet look like halo (cf pictures) and I have no idea why. Anyone has the solution ? I use the protocol and reagents from biotechne :...

24 June 2024 4,184 0 View

What are the key factors influencing the adoption and implementation of Building Information Modeling (BIM) in sustainable construction projects?

What are the key factors influencing the adoption and implementation of Building Information Modeling (BIM) in sustainable construction projects, and how do these factors impact project...

14 June 2024 202 4 View

How do financial ratios influence decision-making processes in construction management?

Financial ratios are critical tools in assessing the financial health and performance of construction projects and companies. Understanding their influence on decision-making processes is...

14 June 2024 1,639 3 View

How can zoning policies and urban development regulations be optimized to promote vertical development and enhance land use efficiency?

Understanding how zoning policies and urban development regulations can be optimized to promote vertical development and enhance land use efficiency is crucial for addressing the challenges of...

14 June 2024 6,311 12 View

What is urban noise pollution abatement management in details?

I need a clear and concise explanation

06 June 2024 6,682 3 View

Exist design guidelines for full-scale aerobic granular sludge process?

Are there any (national) technical guidelines for the process design of a full-scale water resource recovery facility applying the aerobic granular sludge process? The guideline should emphasize...

30 May 2024 9,287 1 View

How project management is impoertant in the development of the country?

22 May 2024 3,480 2 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Issues with Protein Expression in Transfected cells?

Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...

30 July 2024 6,648 1 View

Does anyone have experience electroporating SKOV3 cells with a large plasmid using Neon transfection?

I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?

29 July 2024 3,229 1 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

What is the best way to drop a plasmid?

Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...

25 July 2024 2,532 3 View

Isolation of PLV6-Bmal-Luc plasmid?

I am currently at a stage in my research where I will be using the pLV6-Bmal-luc plasmid (Addgene #: 68833). However, I am encountering some issues with the isolation of this plasmid and am unable...

23 July 2024 5,296 0 View