Is anyone familiar with extraction of genomic DNA?

More Vabeiryureilai Mathipi's questions See All

Which solvent system will be good fixative for adherent culture cells?

I have performed Micronucleus assay after treated adherent HeLa and A431 cells for specific time. The cells were trypsinized into single cell, centrifuged @ 500rpm for 5 min. Fixed with Carnoys...

14 February 2016 126 3 View

How acurate is Acridine orange/ ethidium bromide detection of apotosis and micronuclei?

I am working on plant based compound activity on human cancer cell lines. I would like to observe any damage to DNA but i am confused what experiment will be appropriate. Can anyone share me...

06 February 2016 850 4 View

Has anybody used imageJ software for Gel analysis?

How reliable is Image J for quantification of protein and analysis of Gel image (jpeg,tiff)? Has anyone used this software ever? Please kindly suggest any software used for quantification of...

18 January 2016 168 3 View

Can anyone help me calculate Phenol content of Plants extracts?

I need to calculate equivalent of phenols contents from plants extracts. But confusing after reading related questions on this site. Kindly provide me possible the solution to below query: I have...

24 May 2015 4,523 9 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Jason P Beech

Could you specify - How long would you like the fragments to be? Thanks.

Shobha Kumari

You can use qiagen genomic DNA extraction kit, they give full and very easy protocol.

good luck

Ingo Schroedter

If you have 5 to 10 million cells DNAzol is an option.

I would not use it if you had less cells.

Hadjkacem Basma

you can use Trizol.