I thoroughly pipette my cells prior to electroporating so they should form a monolayer when plated. Though every time I electroporate, they clump. I'm not entirely sure why. When I passage, they plate just fine. Has anyone had an issue with cell clumpiness following electroporation, and if so is there anything I can do about it?
Ajay Saini
Hi Martin,
We routinely do electroportaion of E. coli strains, but we don't see such problem..Are you also talking about bacterial cells? some particular strain or some other cell type?
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Martin Forde
Mammalian, SH-SY5Y. I think it might be a viability issue, since I've tried a different protocol and the cells look more dispersed throughout the plate.
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