Is there a dataset of atmospheric river tracks for the whole continental US?

I'm looking for the period 1960-2010, so the dataset would most likely have to be based on re-analysis data. Most things I found are for the West Coast only and often just feature the landfalling...

18 January 2021 3,466 2 View

How is single molecule's fluorescence lifetime depending on excitation density?

It is known, that the fluorescence lifetime of an ensemble of molecules being in close vicinity can change, as intermolecular processes between excited chromophores (e.g. superradiance,...

28 October 2020 7,656 3 View

Minimum participants for a focus group interview ?

I am involved in qualitative research and at the time working on the methodological background. My colleague, which was doing the interviews, brought to me they did some focus group interviews....

30 March 2020 4,000 16 View

No title

10 June 2019 6,662 4 View

How to mesure fluorescence excitation intensity under microscope ?

Hi everyone, My problem is quite simple: I would like to get the fluorescence excitation intensity in mol.m-2.s-1 (or W.m-2). And I didn't find any protocol/advice online to measure it. I...

15 January 2019 9,583 2 View

What insulin receptor antibodies do you recommend for WB and ICC on rat neurons (Santa Cruz sc-710 and sc-711 aren't sold anymore)?

My stock of sc-711 is gone, and SC has been prohibited from selling polyclonal antibodies. I want to do both WB (could be either IRalpha or IRbeta) and ICC, especially unpermeabilized cells (so...

15 January 2018 2,474 3 View

Who knows talented prospective PhD students who want to pursue an academic career in sustainability science?

27 April 2017 2,147 2 View

Do you know of studies that compare the proactiveness of environmental strategies of suppliers with those of OEMs (in the same sector)?

28 April 2016 1,121 1 View

Which method should we use for analyzing a sample of 45 companies at two points in time?

25 February 2016 1,868 3 View

Can anyone help with co-localization using confocal laser scanning microscopy?

Dear all, I try to identify the cell type (lamina propria) bearing a receptor which I stained (DAB, and AlexaFluor-546) on paraffin-embedded human colonic tissue. Previous co-localizations of...

14 August 2014 447 4 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

Phenol-pH graphic?

We want to use phenol pH graphic to measure the pH of mediums before and after inhibitor application to cells. We have phenol absorbence values at 460, 560 and 720 nm wavelength. When we measure...

20 February 2021 7,700 3 View

Which way will be better to give OGT inhibitors doses through intravenous or orally for pregnant female rats and concentration suggestion?

I'm planning to use ST078925 OGT inhibitor from TimTec (OGT-O-linked N-acetylglucosamine (GlcNAc) transferase) for female rats.

16 February 2021 7,850 2 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Is there a way in which microglia can be extracted from mice without killing them ?

I'm aiming to use an adoptive cell transfer approach, with microglia in mice with ALS. So far all the protocols I have seen, require sacrificing the mouse. I'd like to re-infuse the microglia back...

11 February 2021 9,509 1 View