I am trying to figure out a way to have cortical slices with detectable only the SST Martinotti cells. And if possible a way (with virus) to optogenetically activate only these inhibitory projections to principal neurons. I would appreciate any help in this matter. Thank you.
Thank you for your immediate reply. I have extensively checked the literature, but i couldn't find SST-Cre line for Martinotti cells. All these lines study SOMs neurons in general. I was thinking if i could breed SST-FloP line (SSt is in all SOMs) with CR-Cre line (Calreritin is expressed in cortical Martinotti) and the F1 generation with SST/CR could give the desirable Martinotti neurons. But again i need to label them with fluorescence. I am so confused with all these Jackson's lines.
The GIN mouse line (JAX 003718; Oliva Schwann JNeurosci 2000) exclusively labels Martinotti cells (MCs) in layer 5 of visual cortex, see Buchanan et al Neuron (2012) 75:451. It is a very sparse line, GAD67-based so post-hoc characterised as MC specific, it only gives you the tag, not the ChR2, and the genetic background might not be the best (albino), so perhaps not ideal for your purposes. To my knowledge, there is no Cre line specific for MCs. Any Sst-Cre line will drive at last three cell types, see McGarry Yuste Front Neural Circuits 2010, doi: 10.3389/fncir.2010.00012. I suggest the GIN line + patching + post-hoc verification with morphology and spiking pattern to achieve the best specificity for MCs.
Thank you Per Jesper. I found way with triple breedings to produce the MC mouse line. I just try to figure out if i can order a virus with ChR2 specific for this line.
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