How to preserve endogenous GFP/RFP during IHC?

I have a dual reporter mouse that expresses RFP and GFP under two promoters. I would like to also do IHC on this tissue but the antigen retrieval step (heat) denatures the GFP. Moreover, if I skip...

11 December 2019 403 3 View

Overexpression of a Fe-S cluster protein in E coli?

I would like to achieve heterologous overexpression of a mycobacterial enzyme in E coli that harbors a Fe-S cluster. Unfortunately, the expressed and purified His-tagged protein apparently lacked...

03 May 2016 8,643 4 View

Can sodium polytungstate dissolve soil organic matter during fractionation?

Application of sodium polytungstate is a widely used method for separation of soil aggregates based on their density (eg. Zimmermann et al. 2007). But during the centrifugation process the color...

09 March 2016 761 3 View

Can someone write me how I can decode the exe.stackdump?

I use Cygwin64 linux emulator on Windows 7. After I had run an f.90 code I got the SIGABRT:Process abort signal message. I would like get to know from exe.stackdump file what causes this issue....

10 November 2014 5,865 0 View

Could someone offer me a software in which I can analyse tephigram and emagram?

I look for software where I can calculate Saunder`s fog point from rawinsounde data for Stratus forecasting? I know about RAOB software but I would like to know if there is anything else suitable...

07 August 2014 6,879 0 View

Does anybody have experience with cdo installation to UBUNTU 14.04?

I would like to use cdo in UBUNTU 14.04. I built and installed the required libraries (zlib-1.2.8, hdf5-1.8.12,grib_api-1.9.18, jasper-1.900.1, netcdf-4.2.1.1, cdo-1.6.4rc4) by the following...

08 May 2014 410 4 View

Does anybody have experience with cdo installation to UBUNTU 14.04?

I would like to use cdo in UBUNTU 14.04. I built and installed the required libraries (zlib-1.2.8, hdf5-1.8.12,grib_api-1.9.18, jasper-1.900.1, netcdf-4.2.1.1, cdo-1.6.4rc4) by the following...

04 May 2014 7,959 4 View

Must subscript indices either be real positive integers or logicals?

I would like to calculate the standard deviation of cloud droplets diameters which are between 4.24-53.43 micrometers. When I use the std(x) function of Matlab I get the following error...

04 May 2013 6,124 0 View

I used the lognpdf(x, mu, sigma) function in Matlab 7.11.0 (R2010b). The value of x, mu and sigma were both real data.

03 April 2013 1,808 0 View

No title

I am a student from Babes Bolyai University-Romania, and I have a study and I cannot continue without this scoring template. I don't have possibilities to buy the template. The test was bought by...

06 March 2013 7,355 1 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View