How to preserve endogenous GFP/RFP during IHC?

I have a dual reporter mouse that expresses RFP and GFP under two promoters. I would like to also do IHC on this tissue but the antigen retrieval step (heat) denatures the GFP. Moreover, if I skip...

11 December 2019 403 3 View

What are possible reasons for a massive baseline drift (hill) in denatured ESI-MS with human serum albumin?

We experience a massive baseline drift, it more or less looks like a hill, when injecting human serum albumin purified from Pichia pastoris in a Q-TOF SYNAPT G2 HDMS (Waters). I have attached a...

14 April 2016 9,448 4 View

Is anyone aware of a complex database of ARM Cortex M processors?

Is anyone aware of an overview database of ARM Cortex M processors for most licensed manufacturers, listing Flash, ROM, clock etc? I'm trying to figure out common specification classes.

16 October 2015 1,522 1 View

Sum formula annotation in high-resolution mass spectrometry using isotopic abundances?

I'm interested in doing sum formula annotation to highly resolved metabolites in high-resolution mass spectrometry via the isotopic peaks. A nice summary of how this is done I've found...

13 February 2015 824 7 View

Can someone write me how I can decode the exe.stackdump?

I use Cygwin64 linux emulator on Windows 7. After I had run an f.90 code I got the SIGABRT:Process abort signal message. I would like get to know from exe.stackdump file what causes this issue....

10 November 2014 5,865 0 View

Could someone offer me a software in which I can analyse tephigram and emagram?

I look for software where I can calculate Saunder`s fog point from rawinsounde data for Stratus forecasting? I know about RAOB software but I would like to know if there is anything else suitable...

07 August 2014 6,879 0 View

Does anybody have experience with cdo installation to UBUNTU 14.04?

I would like to use cdo in UBUNTU 14.04. I built and installed the required libraries (zlib-1.2.8, hdf5-1.8.12,grib_api-1.9.18, jasper-1.900.1, netcdf-4.2.1.1, cdo-1.6.4rc4) by the following...

08 May 2014 410 4 View

Does anybody have experience with cdo installation to UBUNTU 14.04?

I would like to use cdo in UBUNTU 14.04. I built and installed the required libraries (zlib-1.2.8, hdf5-1.8.12,grib_api-1.9.18, jasper-1.900.1, netcdf-4.2.1.1, cdo-1.6.4rc4) by the following...

04 May 2014 7,959 4 View

Must subscript indices either be real positive integers or logicals?

I would like to calculate the standard deviation of cloud droplets diameters which are between 4.24-53.43 micrometers. When I use the std(x) function of Matlab I get the following error...

04 May 2013 6,124 0 View

I used the lognpdf(x, mu, sigma) function in Matlab 7.11.0 (R2010b). The value of x, mu and sigma were both real data.

03 April 2013 1,808 0 View

What does Servant Leadership mean today and in the future? What are the advantages and disadvantages?

In current time of many changes in business and world of work, a lot is said and reported about the importance, skills and benefits of leadership. Are soft skills becoming more and more important?...

02 March 2021 8,149 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Can I download BibExcel for MacOs?

Hi everyone, I would like to ask how I can download BibExcel software for MacOs? There are some links which are supported by Windows but I could not find it for Mac. or is there any other software...

01 March 2021 652 1 View

What role should journals play in leading academic fields?

A recent paper by Yeung and Thomas (2021) on journals in event management opens up an interesting debate on the role of journals as academic fora. They demonstrate that event management publishing...

28 February 2021 3,494 6 View

Anyone with some Transgenomic Wavemaker software? Seeking help from the chromatography community?

I am trying to seek out some Transgenomic Wavemaker software (happy to purchase a computer too) to operate a fraction collector. The only way to connect to the fraction collector so far is to...

23 February 2021 9,181 1 View

Best excel chart to compare algorithms' running time?

Hi I want to compare the running time of robotic path planning algorithms. The grid size is 8X8. I have 100 scenarios of start and goal positions.. Each scenario is run in three obstacles ratio...

22 February 2021 621 1 View

Can I follow Researchers from Teaching and testing areas?

I want Researchers from Educational Measurement and Evaluation, relating to teaching, learning , academic performance and test validation

18 February 2021 4,757 13 View

How to open an old ".MTS" file containing spectroscopic data?

Hi supreme hive-mind of the internet, we have some old *.MTS files that are supposed to contain spectroscopic data, probably some coordinates and a long list of single line spectra. Basically,...

16 February 2021 9,385 2 View

Room Occupancy Calculation ?

15 February 2021 1,997 6 View

How to check if the PMF profile obtained from ABF simulation is correct?

I am doing ABF simulations to calculate ligand binding enegy. The PMF profile that I get using single long ABF simulation is attached as pmf_total.png. When I do this ABF calculation with 10...

10 February 2021 4,170 2 View