Does anyone know of a method that can reliably deplete all hemoglobin-bound and free oxygen content of the blood ex vivo? I need to create a sample of oxygen free blood in a sampling tube or syringe. It does not need to be "life compatible", since it will not come into contact with a living system again, however it is necessary that all oxygen content is removed.
Depending on the application, you may want to look at using yeast (brewer's yeast is easily available) or sodium dithionite.
Depending on the application
you can add sodium metabisulphite, diluted with PBS, to (RB-)cells ; Na2S2O5, Molmassa190,10654 g/mol
or
you can use the Lorrca Oxygenscan ektacytometer to study de-oxygenation and re-oxygenation of RBC in shear stress (automated test).
Try to use Carbonmonixide or any other substance that dispalces teh oxygen from the hemoglobin.
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