Is anybody using protocol for staining the cells using acridine orange or MDC for autophagic detection through flow cytometry?

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Senescence-associated beta galactosidase staining is False Positive in control group?

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Subhadeep Roy

We have done compartment trafficking and autophagy detection in our lab on regular basis. For flowcytometric analysis and qualifications you can use the following kit.

http://www.enzolifesciences.com/ENZ-51031/cyto-id-autophagy-detection-kit/.

It will fulfill your requirements.

Ramachandramouli Budida

I agree with Subhadeep. We also used the same kit for Autophagy measurements using Flow cytometry and microscopy. Please find the attached article. Thanks

Article Herpes Simplex Virus 1 interferes with autophagy of murine d...

Sameer Tiwari

Thanks, Subhadeep for the link you provided to determine autophagy.