Hello Julia, I personally did not have a good experience with the EasySep kit from Stemcell Tech. I cultured Ovarian Carcinoma cell lines together with CD14+ monocytes, and tried to harvest the monocytes afterwards. Using the EasySep was not very effective. On the contrary, using Magnisort MACS beads and respective a-CD14 from Affymetrix-eBioscience resulted in very pure isolates as detected by flow cytometry, (90+% if I recall correctly).