I want to detect lipophosphatidic acid in plasma or serum sample. What is the exact assay methodology or a refernce for doing this?
Hi! I am using nested PCR approach to detect mucosal HPV in cervical specimens as well as from FFPE tissues. This is to increase the sensitivity. But in doing so, I am getting a same similar size...
02 March 2018 5,965 5 View
HPV oncogene E6/E7 EXPRESSION DETERMINES THE ONCOGEENIC POTENTIAL OF THE PRESENT VIRUS. However, I wanted to know the assays which are available to detect E6/E7 mRNA expression, would they be...
08 September 2015 8,922 3 View
I am extracting viral RNA from a human tissue. I have DNA elute. Now if I give DNASe 1 treatment, how do I proceed with further elution of DNA free RNA?
07 August 2015 5,338 4 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,526 4 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I went for Plackett Burman Design for selecting the optimization factors of growth conditions for pesticide degradation. I started with 7 parameters. But, after the experiments and analysis, only...
29 July 2024 5,015 1 View
Contemporary scores or innovations in scientific approaches. Hybrid methodologies, emerging themes and cross-cutting issues?
27 July 2024 648 3 View
I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...
24 July 2024 5,789 0 View
I am looking for journals that admit the publication of results from applying the IUCN Standard for designing and monitoring Nature-based solutions. Many thanks for considering my request.
23 July 2024 6,444 2 View