the egg contents will be pretty sterile I'd think. Why not do the whole thing in a cell culture hood? Wash off the outside of the egg with ethanol, open it and use Sterile PBS to dilute it, then innocculate that? Should be much more straightforward than worrying about inefficient UV sterilisation, or (even worse) trying to filter sterilize something as viscous.

Good Luck

G

UV is not very penetrating as a sterilizing agent, and even if you make the eggs thin enough for penetration (like a thin layer) it will not remove any contaminating DNA.

Thanks to you all, egg content is not sterile, I have already isolated bacteria from the content, and previous researchers also did. A vertical transmission of bacteria during the egg formulation might occur in infected hens.

My problem is: did you mention an intensity or a dose, what was the amount you applicated? What kind of UV did you applicate UVA, UVB, UVC?

I know from dependencies for ionizing radiations from dose, energy.... What about these facts for UV.

I´m also missing hints to the grade of sterilisation. You never can sterilize to zero rest bacteria.