I have been using 27f and 1492r primes to sequence 16s region form Burkholderia spp. from rice samples. Since the Burkholderia complex species are very closely related I have been wodering if 16s region is enough to build a phylogenetic tree.
Thank you Dr. Spencer. I will definitely check those resources. I did not know about RDP. Today I am aligning recA gene regions from different Burkholderia spp available on GeneBank since some authors have reported it is useful to differentiate closely related bacteria.