Carlos Antonio Riera-Ruiz

9 Questions 10 Answers 0 Followers

Questions related from Carlos Antonio Riera-Ruiz

Filtering 5M NaCl through 0.02 um membrane. Will it change final concentration?

I made a 5M NaCl solution and tried to pass it through a 0.02 anotop filter. After the first 10 ml, the filter seem to have clogged. Is NaCl somehow crystallizing and clogging the filters? Which...

06 August 2021 4,651 0 View

What is the rationale behind overproducing a protein as a fusion instead of as a single protein?

I'm reading a paper about CRISPR and they say: "To investigate whether the CasE subunit is sufficient for pre-crRNA cleavage activity, it was overproduced as a fusion with the E. coli maltose...

25 November 2018 8,853 4 View

Is there an endophytic microbiome in commercial corn seeds form the USA?

I have no knowledge about how the seed production is done in the USA and I'm wondering whether the microbiome of the commercial corn seeds is worth to study or is not as diverse due to the...

01 November 2018 6,261 2 View

Any fasta to Phylip relaxed conversion program?

I have been using readseq. Although it provides interleaved and sequential Phylip format I need the relaxed sequential phylip format to see the complete organism's name.

27 November 2017 2,569 8 View

Is 1Gb of sequence data enough to assemble a bacterial complete genome?

A company is offering 1Gb for $xxx. Since it is supposed that 1Gb=10^9 bases, is it enough for assemble a whole bacterial genome (~9Mb)?

14 November 2016 377 4 View

What regions of bacteria chromosome shoud I sequence in order to design species-specific primers?

I'm working with Burkholderia spp. from rice samples. I need to identify them for rapid diagnosis but reported primers are not working with my samples. I know it is unusual, that is why I decided...

22 February 2016 7,887 3 View

Is 16s region of bacteria chromosome enough to build a phylogenetic tree of Burkholderia spp. from rice samples?

I have been using 27f and 1492r primes to sequence 16s region form Burkholderia spp. from rice samples. Since the Burkholderia complex species are very closely related I have been wodering if 16s...

22 February 2016 541 1 View

What factor or compounds could induce overproduction of hormones in plants?

I'm studying the effect of craft organic products in rice.

20 March 2015 4,741 3 View

What method is optimal for preparing a rice tissue sample (leaves, stems and root) for cut in a microtome?

I want to observe inoculated bacteria on rice tissue. Some bacteria were tagged with gfp while other with bfp.

08 September 2014 5,748 3 View