Carlos Antonio Riera-Ruiz

6 Questions 9 Answers 0 Followers

Questions related from Carlos Antonio Riera-Ruiz

What is the rationale behind overproducing a protein as a fusion instead of as a single protein?

I'm reading a paper about CRISPR and they say: "To investigate whether the CasE subunit is sufficient for pre-crRNA cleavage activity, it was overproduced as a fusion with the E. coli maltose...

25 November 2018 8,797 4 View

Any fasta to Phylip relaxed conversion program?

I have been using readseq. Although it provides interleaved and sequential Phylip format I need the relaxed sequential phylip format to see the complete organism's name.

27 November 2017 2,506 8 View

Is 1Gb of sequence data enough to assemble a bacterial complete genome?

A company is offering 1Gb for $xxx. Since it is supposed that 1Gb=10^9 bases, is it enough for assemble a whole bacterial genome (~9Mb)?

14 November 2016 309 4 View

What regions of bacteria chromosome shoud I sequence in order to design species-specific primers?

I'm working with Burkholderia spp. from rice samples. I need to identify them for rapid diagnosis but reported primers are not working with my samples. I know it is unusual, that is why I decided...

22 February 2016 7,830 3 View

Is 16s region of bacteria chromosome enough to build a phylogenetic tree of Burkholderia spp. from rice samples?

I have been using 27f and 1492r primes to sequence 16s region form Burkholderia spp. from rice samples. Since the Burkholderia complex species are very closely related I have been wodering if 16s...

22 February 2016 481 1 View

What method is optimal for preparing a rice tissue sample (leaves, stems and root) for cut in a microtome?

I want to observe inoculated bacteria on rice tissue. Some bacteria were tagged with gfp while other with bfp.

08 September 2014 5,658 3 View