What are the Hypotheses can i frame for the topic " Social media as a Source of Information to Engineering Students"?

i am planning to do research on the topic " Social media as a Source of Information to Engineering Students" for this please share valuable thoughts.. which type of Hypotheses can suitable for...

27 October 2018 8,864 3 View

Can okra plant be propagated by stem cuttings?

Dear All, I have identified a putative male sterile line in okra(Abelmoschus esculentus- through wide hybridization) (confirmed sterility with crossing/ selfing/ pollen studies). In order to...

10 October 2017 7,706 3 View

Can you suggest alternate chemical for potassium tetraborate k2b4o7 which is essential for chitinase assay?

Can you suggest alternate chemical for potassium tetraborate k2b4o7 which is essential for chitinase assay?

20 December 2016 2,182 3 View

Clone having repetitive sequence insert and its discripancy upon scale up?

I have cloned my 3.3Kb insert having repetitive sequence in yeast expression vector, by directional cloning in E.coli. Positive Clone of 8.7Kb was confirmed by restriction analysis. While the same...

26 January 2016 4,477 2 View

How can I implement a indirect matrix converter controller?

electrical engineering

11 February 2015 6,989 3 View

What are the strong reduction agents to remove the epoxy(=O) groups and -COOH groups?

what are the different reducing methods and reduction chemicals...

24 August 2014 2,993 3 View

Comparison of Nano drop and RNA integrity number (RIN) data?

I have isolated total RNA from mammalian cell culture samples. I used a Qiagen Kit. I have checked the conc and quality of RNA by Nanodrop. Ratio of 260/280 was between 2.01 and 2.09, and 260/230...

21 August 2013 4,011 16 View

mRNA level and its comparison to protein level.

I am measuring a gene which is involved in differentiation of a cell. During differentiation its level is high, compared to undifferentiated state. Upon terminal differentiation of cell, mRNA...

20 June 2013 2,588 2 View

suggestions for using alternate pcr method for race

hello friends! any one suggest me alternate pcr method for plant cloning and expression instead of using race..

25 July 2011 3,681 1 View

Chromosomal counting in okra species?

I have been trying to prepare root tips for chromosome counting studies in okra (Abelmoschus), but every time I am failing to get stained root tips with Feulgen stain. The root tip portion is not...

01 January 1970 5,105 4 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

No title

02 March 2021 3,060 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View