What are the effects of longer exposure to siRNA (for KD)?

1) I knocked down a protein and collected cells 24 h and 72 h after transfection with Lipofectamine (to note, 5 h after transfection medium was replaced with medium with FBS). 2) KD efficiency...

08 December 2020 9,850 1 View

Why is hard to produce antibodies that are specific to a protein?

Some antibodies reveal nonspecific bands when a western blot is performed. Why is hard to find a good antibody for a protein? Antibodies produced using as the immunogen a synthetic peptide or a...

03 December 2020 208 3 View

Why different antibodies for the same protein stain different places?

I tried two antibodies and one show stain in the cytoplasm and the other one in the nucleus. Why? (Blue - dapi; red - mitochondria; green - protein of interest) And why is hard to find a good...

24 November 2020 6,612 7 View

Do you have experience visualising multiplex amplification curves in LightCycler 480 Sw??

We are using Cobas z480 for multiplex RT-PCR using 3 taqman probes FAM/VIX/CY5. Once the PCR finish we want to see in the amplification curve the three results for each sample at the same time....

28 October 2020 8,372 1 View

TEACHING ESL in the lockdown - participants needed?

Dear English lecturers (teachers, instructors), I am conducting research into ESL teaching to increase our understanding of how teachers have experienced special teaching conditions during the...

14 October 2020 7,223 6 View

Why does mitotracker red stains the nucleus?

I'm working with NT2 cells and I use mitrotracker red (400 nM) to stain the mitochondria. I incubate it with cell medium for 30 min, fix with 4% PFA, permeabilize with PBST (0.2%-0.5% Triton...

11 August 2020 8,076 3 View

What psychometric properties should be reported for a translated version of a questionnaire?

Dear collegues, I performed a cross-cultural study using two questionnaires (66 and 12 items). The original version of these questionnaires was used in the first country, and these tools were...

27 April 2020 4,097 9 View

USRP N210_ RF1 and RF2 dont work ?

Hi, I am working on USRP N210 and Matlab 2016R. I want a transfer a Sinusoidal wave from one port of Antenna and want to receive same wave from second Antenna at one USRP N210 (unfortinaly I...

28 January 2020 5,568 3 View

Problem in modulating miRNA expression (both over-expression and knock-down) in mouse cell lines?

Hi everybody, we are working with let-7 family and we would like to perform knock-down of the let-7 using Lin28 expression vector. In another experiment we would like to over-express let-7g from a...

20 September 2017 9,420 2 View

Can anyone help with a PCR insertion/deletion genotyping problem?

09 July 2014 4,184 3 View

No title

02 March 2021 932 1 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

How Download/Extract citations of 1 given year ALL my publications in Google Scholar?

If you google this you find a lot of smart guys explaining you how to download 1 year of citations of 1 single paper of yours (or anyone else). I mean 1 year of ALL my papers. or ... ALL years or...

01 March 2021 1,121 9 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View

Could anyone tell me what is the range of submillimeter wave?

There is confusion regarding the submillimeter-wave range. Many papers specify its range from 300GHz but some also consider its range from 100GHz. If I design an antenna on 122ghz what would I...

01 March 2021 516 7 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View