I am trying to work out a protocol for measuring sodium hydrogen exchange activity by using a pH sensitive dye BCECF-AM, I am planning on using the BMB NOVOSTAR plate reader for measuring the flourecenceof BCECF-AM. I was wondering if anyone would be willing to share ther protocol as well as the settings on the BMG NOVOSTAR plate reader for intarcelluar pH measurement using BCECF-AM

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