Your 'N' in all entries has no band, except entry 'kwan' that has a faint band. I guess the 'N' is a negative control . 'S' in all entries except G1/A has a single band (1 kb). So, what is the difference between G1/A sample and other samples?
As Sudip Das said, you need to explain a bit of what your goal of your experiment, so people can give a better diagnosis and find out whether the gel results make sense.
Its extraction of DNA from bacterial sample and using gel electrophoresis to determine the size...Yuan you are correct that n is negative control
S S means one sample for two replications. All are same sample just done by different people. I want to knw why is thr variation in electrophoresis even after doing same sample and using same extracted DNA?
@ Sushil Koirala,
Yes, now you have given a very good description of the purpose of running this gel and the questions you really want to know ("The variation in electrophoresis even after doing same sample and using same extracted DNA?").
PCR is a very sensitive tool. Different results can be due to many factors. In your case, for example, different people can take up different amount of DNA template from the same stock tube, different people can also drop a different amount of DNA template into a PCR reaction tube. This variation is from DNA sample amount. If different people prepare their own PCR components (ex. dNTPs, MgCl2,....), another variation will be there. Let along the stock DNA sample solution might not be homogenous, depending on how well the isolated DNA pellet is dissolved. Different PCR machines can also contribute different PCR results. The running positions on the gel can also display different intensity of the PCR product (in your case, run on the top part of the gel vs. lower part of the gel).........
Can i interpret that i can reduce my elution buffer to get more clear band or increase the intensity of band?
What does 16S represent on the figure.
My applied potential was 150V will it affect result
i used safe stain and not bromide stain?
@ Sushil Koirala,
For your first question, I have to be very careful to answer. In theory, yes, and assume your DNA purity is good, and no PCR inhibitors and such.
For your second question, it is funny. I thought this is your gel and you should've known what 16S stands for. I am not sure, but I guess that it is the gene of 16S ribosomal RNA (or 16S rRNA) which is the component of the 30S small subunit of a prokaryotic ribosome
150V, you can run it slower. Besides, your 100 bp DNA markers was very burry. It hardly gave you accurate size of your DNA fragment.
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