Wisdom is understanding how to apply Knowledge in the real world. What is your wisdom in life?

Dear All Wisdom is understanding how to apply Knowledge in the real world. Wisdom is cultivated by following Knowledge and by developing skills in the world. It is based upon the cultivation of...

01 February 2017 7,924 35 View

In which case the Kubelka-Munk equation is used

31 December 2016 1,486 5 View

What You know about the Shatt Al-Arab River? Do you have a description of Shatt Al-Arab?

31 December 2016 5,412 13 View

Health education is a profession of educating people about health. What do you think?

Health education is any combination of learning experiences designed to help individuals and communities improve their health, by increasing their knowledge or influencing their attitudes.

10 November 2016 2,301 0 View

How can the parasites grow larger in faster growing fish hosts?

I need some information about the parasites grow and fish hosts.

10 November 2016 6,886 12 View

What the practical considerations relating to the health of your fish in the real world of aquarium keeping?

Although one may think otherwise, the problems our fish encounter inan aquarium are not the same as they would encounter in the wild.Although the parasites and bacteria will be the same, in most...

10 November 2016 9,039 0 View

How can to be effect of pollutants on Condition Index in fish?

10 November 2016 1,473 5 View

What are the best serological parameters in fish, dietary prebiotic?

10 November 2016 3,666 2 View

How can the dietary modulation of the colonic microbiota when used a prebiotic in fishes?

10 November 2016 9,459 2 View

What are the Physiological changes in fish from stress in aquaculture?

Physiological stress and physical injury are the primary contributing factors of fish disease and mortality in aquaculture. Many potential fish disease pathogens are continually present in the...

10 November 2016 363 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Hi i want to ask that in order to find out LC 50 (96 hours) of particular toxin for fish. Should we keep the animals with food or without food?

if we expose the fish after acclimatization to particular toxin in order to find out LC 50 (96 hours).Should we keep the fish off feed or with feed for that duration. Some literature show that...

24 February 2021 357 2 View