Defining mixed model with repeated measures data?

HI all, I have been working with daily diaries data ( 6 emotions of which 3 positive and 3 negative) were assessed for 30 days. These observations (1level data) are then nested within subjects...

13 February 2021 1,810 3 View

High percentage agarose gel for better resolution?

Hi all, it might be a very basic question, but I need some opinions: I need to separate a 294 bp wild type PCR product from a 232 bp knock-out PCR product. I want to do DNA extraction out of the...

08 June 2020 9,557 9 View

What do you think of the impact of Covid-19 on faster acceptance of innovative healthcare technologies?

After this pandemic outbreak, nothing will be the same. Previously suspicious attitude toward accepting innovative solutions in clinical practice and long times for regulatory procedures and...

09 April 2020 7,691 10 View

Any experience with Health Informatics Journal?

We would like to publish something which is in the domain of Health Informatics, but several Journals have almost identical names. any suggestions, experience with publishing with those Journals...

22 February 2020 7,635 9 View

Do we need a standardization prior to publication of any machine learning application?

What do you think about so high accuracies reported in so many publications especially in medicine and psychiatry? Talking about overfitting and so frequent lack of internal and external...

07 October 2019 2,558 4 View

Is it possible to stain cells with Acridine orange/ethidium bromide in hydrogel 3D invasion assay?

I am planning to perform 3D invasion assay with spheroids in hydrogel and to visualise live/dead cells. Is it possible to visualise cells with AO/EB (besides CAM/EtHD) dyes? Thanks

18 October 2018 5,722 3 View

Can LPS be captivated by the tube if you freeze and subsequently thaw it?

15 January 2017 7,051 3 View

Does someone can recommend a good Elisa kit for mouse NTx (N-terminal telopeptide ) ?

Hello everyone, I need to measure NTx (N-terminal telopeptide) marker in a mouse serum. I would really appreciate if someone could share experience with this kind of ELISA kit, most importantly...

10 April 2016 5,458 1 View

What is wrong with my intestinal organoids?

I am trying to grow swine intestinal organoids from crypt preps. I have been doing it with mouse tissue for at least a year now. For the pigs, a few days after getting the crypts, there are...

05 April 2016 3,294 7 View

Does anyone know how to create nucleotide and amino acid deduced sequence visualization in an automated way?

I would like to generate a visualization similar to that you can see in the attached image (Mu et al., 2012, Fish and Shellfish Immunology Journal).

15 September 2012 7,903 6 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Which reagent is best among 'Dimethylglyoxime" and "Dithiothreitol" for spectroscopic determination of Nickel ?

What I think is Dithiothreitol. As it is water-soluble and does not form a precipitate. But, the thing is it contains sulphur which is quite hazardous to aquatic animals.

01 March 2021 8,374 4 View

How to identify what type of fillers are in a polymer?

Hello everyone, I have some PHAs blended with fillers and nucleating agents, yet I don't know what kind of fillers or nucleating agents. The producer has shut down and there's no way to get in...

24 February 2021 1,356 8 View

Is it mandatory to specify Subjects Combinations in Generalized Linear Mixed Models (SPSS)?

Hi all, I have data with two levels: lets think of the classic example of students and schools. When running the analysis in SPSS and selecting the Random Effects, we can select (we can or we...

24 February 2021 7,379 4 View

Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations?

Dear colleagues, Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations? The question is mainly aimed at adolescents (pupils,...

23 February 2021 7,796 4 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

How to minimise the risk of being abused the profile of potential participants tagged in a social media post?

Dear colleagues, I got this one in one project I did ethics review. It's great to hear about your thoughts/experiences. "Doing an open recruitment online (i.e., via Facebook) requires public...

22 February 2021 3,322 3 View

What type of SPSS analyses should I use for my Dissertation?

I'm currently working on my Dissertation and I'm trying to think of the best analyses that I should use for my study. I'm using three questionnaires for this and two different groups, 1st group...

22 February 2021 1,351 4 View