How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

Do you consider it is important to vindicate Spanish as language, in scientific issues and publication of scientific articles?

03 February 2021 3,756 4 View

I'm curious whether there is any research available on the use of AI related to diversity&inclusion (efforts)?

I'm looking for studies or conceptual papers on the (potential) uses of AI for enhancing diversity and inclusion within organization. The biases concerning the use of (big) data are known and...

18 January 2021 4,222 4 View

Do you know what happen in reaction between Ni2SO4.6H2O and DMG (dimethylgolyoxin)?

I want to use DMG as a reagent for detecting Ni in a solution. in their reaction they form a complex together which color is pink. I attached a picture of the reaction. my problem is I can`t find...

01 January 2021 7,200 3 View

The saturation point does not reach in my ITC. Does this indicate that the interaction is weak or there is no interaction?

21 December 2020 9,407 8 View

What could be the impediments to the initiatives adopted by the bank towards FI in the Indian scenario?

this question from case Union Bank of India: Initiatives towards IT-enabled Financial Inclusion

09 December 2020 7,721 3 View

How to conduct CUSUM and CUSUMsq test after the inclusion of an exogenous dummy variable in my ARDL model?

Hi all, I have been struggling a great deal with this matter and as such I hope you could provide me with answer. I have added a dummy variable to my ARDL model to improve its stability (Eviews)....

01 December 2020 1,071 5 View

What is importance of protein ligand stoichiometry in enzyme activity assays?

I recognize that in a standard enzyme kinetics assay the protein concentration should be negligible, however I have recently come across many assays in which researchers will utilize really high...

09 November 2020 123 4 View

Can we co-relate that A-site vacancies are one of the main reasons for (110)-texture formation? But how?

A-site deficient 0.1BiScO3-BaTiO3 (BSBT) thin films were deposited on Pt/TiO2/SiO2/Si substrate by using PLD. XPS results revealed that A-site vacancies increase with the increase of deposition...

05 November 2020 9,281 1 View

Why the expressed protein band Molecular Weight (MW) is lower than expectation?

I tried to express my protein in BL21(DE3) bacteria. The desired protein MW is 65.5 KDa and I know my protein will be in an insoluble form (inclusion body). I separate soluble and insoluble...

20 October 2020 3,975 8 View