The protein that I tried to expressed in sf9 insect cells is found to be insoluble. I used baculodirect baculovirus expression system, the expression is good, but the problem is that everything is in the pellet. And I don't think lysis is the problem because the pellet of uninfected cells is very clear. Is it normal that insect cell sometimes express inclusion body? And any suggestions for that?
Jan-Hendrik Hehemann
Could it have crystallized in the cells?
Such as in this publication: http://www.nature.com/nmeth/journal/v9/n3/full/nmeth.1859.html
May be worthwhile to investigate, because a crystallized protein could be functional and crystallization opens an alternative route for purification.
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