Most of the primers that are being used for phylogenetic studies of Staphylococcus species are inosine-containing primers (sodA, Hsp60, rpoB, etc.) which make it expensive to order. Any suggestion how can I do my analysis while reducing excessive costs?
Ajay Saini
Hi
Try to re analyze the available sequences once again and redesign the primers in highly conserved region of genes, flanking sufficiently variable region. See if it is possible..if yes you may be able to design simple sets of primers for amplification from different species.
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Omid Shenavar
@Ajay Saini
Thanks for your answer. I'll get it done; hope it works out.
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