Greetings everyone,
How we calculate the inoculum size for mutans streptococci (Streptococcus mutans and Streptococcus sobrinus) bacteria?
I want to know the correct concentration dilutions (cfu/ml) from a 0.5 McFarland preparation of these bacteria.
My problem is; How much my inoculum concentration should be (in cfu/ml), if I want to add 2 ml of this inoculum to 2 ml of the antimicrobial/broth dilutions to get a total of 4 ml with the recommended (5 x 10^5 cfu/ml)?
It seems that there are differences in the studies on the dilution ranges and size of the inoculum for these microorganisms.
Appreciate your kind reply.
Hey,
As I remember the inoculum size of 0.5 McFarland scale for S. mutans equals about 2 x 10^5 CFU/mL. However the best way to find out the size of inoculum is to conduct the seeding on agar plates with serial dilution
Hi Arass,
Use spectrophotometer or
DensiCheck TX is an economic answer to the problem of monitoring liquid concentration and density in a wide variety of applications.
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