The simplest way to determine IC50 value is using a regression equation as follows

                  y=mx+c

I used the above equation and I found IC50 (27.90 micrograms/mL). However, the value calculated manually was found to be (8micrograms/mL) as can be seen in the file attached. Among the two IC50 values, which one to be consider more accurate?

Thank you in advance.

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