Is UV (unit Variance) scaling suitable for FTIR data?

For metabolomic data analysis, different choices for scaling are available such as Pareto, centering, unit variance etc. which of these scaling methods is recommended for FTIR data? Thank you.

07 August 2017 9,880 2 View

How much the standard deviation should be?

07 August 2016 6,098 2 View

Inhibition concentration (IC50). Is the value determined using linear regression equation accurate?

07 August 2016 8,692 2 View

Can i store Potassium Phosphate buffer for long time?

I prepared Potassium Phosphate buffer and i want to be using it for a long time. What are the optimum storage conditions? Thank you

04 May 2016 8,858 5 View

Which solvent is the best option?

For phenolic extraction in a non-defatted sample between methanol, ethanol and ethyl acetate which one will be the best option and why? Thank you.

03 April 2016 6,617 4 View

Please can some one help me with this article title A new method of standartization of health-promoting pomegranate fruit (Punica granatum) extract?

pomegranet

31 December 2015 6,048 1 View

Why do we need to purify plant extract before HPLC Analysis?

I used Three solvent of different polarity ranges from non polar, semi polar and polar, should i need to purify my extract before running HPLC and i need help for the purification protocol. Thank you.

31 December 2015 1,317 10 View

What factors should be considered in changing the composition of mobile phase in gradient elution?

What factors should be considered in changing the composition of mobile phase in gradient elution?

11 December 2015 3,398 4 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View