Antioxidant Assay - FAQS.TIPS

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Is UV (unit Variance) scaling suitable for FTIR data?

For metabolomic data analysis, different choices for scaling are available such as Pareto, centering, unit variance etc. which of these scaling methods is recommended for FTIR data? Thank you.

07 August 2017 10,047 2 View

How much the standard deviation should be?

For a consistent and reliable result, a particular experiment needs to be carried out twice or three times and at the end, the mean value and standard deviation can be calculated. So how much the...

07 August 2016 6,232 2 View

Inhibition concentration (IC50). Is the value determined using linear regression equation accurate?

The simplest way to determine IC50 value is using a regression equation as follows y=mx+c I used the above equation and I found IC50 (27.90 micrograms/mL). However, the value...

07 August 2016 8,890 2 View

Can i store Potassium Phosphate buffer for long time?

I prepared Potassium Phosphate buffer and i want to be using it for a long time. What are the optimum storage conditions? Thank you

04 May 2016 9,089 5 View

Which solvent is the best option?

For phenolic extraction in a non-defatted sample between methanol, ethanol and ethyl acetate which one will be the best option and why? Thank you.

03 April 2016 6,731 4 View

Please can some one help me with this article title A new method of standartization of health-promoting pomegranate fruit (Punica granatum) extract?

pomegranet

31 December 2015 6,167 1 View

Why do we need to purify plant extract before HPLC Analysis?

I used Three solvent of different polarity ranges from non polar, semi polar and polar, should i need to purify my extract before running HPLC and i need help for the purification protocol. Thank you.

31 December 2015 1,479 10 View

What factors should be considered in changing the composition of mobile phase in gradient elution?

11 December 2015 3,524 4 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Measuring the Intelligence of a Species?

Larger brains, which typically contain more neurons, store and transfer more information (Tehovnik and Chen 2015), but the precise relationship between number of neurons and information has yet to...

05 August 2024 1,238 2 View

Determining the worth of a point improvement in Hamilton Depression Scale?

Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...

05 August 2024 3,246 1 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

29 July 2024 4,570 0 View

My cfDNA extraction protocol using silica beads works with plasma collected in COD SAGM bags but not with plasma collected in K2 EDTA tubes. Why so?

I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...

28 July 2024 3,559 0 View

What is the Principle of Cysteine-carbazole-sulfuric acid reaction with glucose?

I have been looking for the principle of Cysteine-carbazole-sulfuric acid reaction in research papers and books, but couldn't find it. This reaction is mainly used for the quantification of...

23 July 2024 3,891 0 View