Is UV (unit Variance) scaling suitable for FTIR data?

For metabolomic data analysis, different choices for scaling are available such as Pareto, centering, unit variance etc. which of these scaling methods is recommended for FTIR data? Thank you.

07 August 2017 9,880 2 View

How much the standard deviation should be?

07 August 2016 6,098 2 View

Inhibition concentration (IC50). Is the value determined using linear regression equation accurate?

07 August 2016 8,692 2 View

Can i store Potassium Phosphate buffer for long time?

I prepared Potassium Phosphate buffer and i want to be using it for a long time. What are the optimum storage conditions? Thank you

04 May 2016 8,858 5 View

Antioxidant Assay

What is the difference between Thiobarbituric acid (TBA) and Thiobarbituric acid reactive species (TBARS) Assay Thank you

04 May 2016 5,934 2 View

Which solvent is the best option?

For phenolic extraction in a non-defatted sample between methanol, ethanol and ethyl acetate which one will be the best option and why? Thank you.

03 April 2016 6,617 4 View

Why do we need to purify plant extract before HPLC Analysis?

I used Three solvent of different polarity ranges from non polar, semi polar and polar, should i need to purify my extract before running HPLC and i need help for the purification protocol. Thank you.

31 December 2015 1,317 10 View

What factors should be considered in changing the composition of mobile phase in gradient elution?

What factors should be considered in changing the composition of mobile phase in gradient elution?

11 December 2015 3,398 4 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View