How long does Hela cancer cells take to reach 80% confluency after thawing from liquid nitrogen?

Three days ago, i thawed Hela cancer cells from liquid nitrogen but until now they are still suspended in the media and very very low number of cells became attached to the tissue culture...

06 January 2021 6,511 4 View

Any recommendations for a good CRISPR/Cas9 commercial system for functional genomics of fungi/yeast?

I look forward to implying a good CRISPR/Cas9 system with a selective maker for functional genomics of important genes from environmental fungi and yeast.

22 December 2020 1,432 2 View

What is the appropriate media for WI-38 cell line?

Iam going to culture WI-38 ( a normal lung cell line), it is the first time for me to deal with this cell line so i want to know the most suitable media for this cell line? In addition to, if i...

18 December 2020 6,542 3 View

Can i use an expired fetal bovine serum?

I have fetal bovine serum expired from many years ago but it kept at -200C during all this period and did not expose to cycles of freezing and thawing. So, can i use it for cell culturing? In...

18 November 2020 1,237 7 View

How can i solve the problem appeared during autogrid run using cygwin?

This is the first time for me to use the autodock software. I used autodock tools 1.5.6 I prepared pdbqt file for my ligand and my target and also file for grid parameters and docking parameters,...

02 September 2020 5,914 2 View

How do you think our Architectural spaces would change after COVID 19?

How do you think our Architectural spaces would change after COVID 19?

10 May 2020 3,172 27 View

When do you increase the number of samples?

Using the short-time Fourier transform (spectrogram) for different sample sizes

19 October 2019 3,799 2 View

statisticaly significant t test results but no fold change?

For example, measuring lymphocytes conc. in control gp was 100 ug/ ml while group 1 average was 115 ug/m, performing student t test results were signficantly different. But how to explain this in...

09 June 2018 7,442 6 View

Softwares to generate simulation data in different distributions ?

I need suggestions of softwares to generate sample sizes with random data in different distributions

14 October 2017 3,743 2 View

What is the theoretical capacity for Na5V12O32 (=Na1.25V3O8) when it will be used as an electrode in batteries?

Batteries Advanced energy materials Energy

31 July 2017 3,715 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View