Hi, i am working on fluorescence microscopy..i am trying to build a small microscope to study fluorescence (eGFP) ..I would like to know if there is any limitation on the excitation time and imaging time. I mean, how long can my excitation source be ON? and for what time period can I capture fluorescence? Is there any delay required between excitation and fluorecence image capture? or, can the excitation light be ON throughout the process?