Hi We have working on Clostridium botulinum in batch fermentation. After the process of fermentation isover we have been doing acid precipitation and collecting the cell debris paste. Later this is solubilized to recover the toxin molecule, however when we do centrifugation after solubilization, very frequently we observe inefficient separation between pellet and supernatant, making it difficult to recover the supernatant. Any suggestions please.
Libor Krásný
Try increasing the centrifugation force (g) or the duration of centrifugation.
0 votes
0 thanks
Ping Wu
Centrifugation Conditions: Ensure that the centrifugation speed and time are optimized. If the pellet is not dense enough, a higher speed or longer time may be required to achieve efficient separation. Pellet Composition: Check if the pellet contains lipids, proteins, or other biomolecules that might interfere with the pellet’s formation. These could alter the pelleting efficiency and make separation more difficult. A pre-centrifugation clarification step may help. Washing Step: Consider adding a wash step before centrifugation to remove any residual solubilized material that may contribute to a loose pellet formation. Density Gradient Centrifugation: If the issue persists, you might consider using a density gradient centrifugation method to help improve the separation and pellet clarity.
It sounds like the issue might be related to either the composition or the density of the pellet, or possibly the centrifugation conditions. Here are a few suggestions:
Hope this helps!
0 votes
0 thanks
- Badges
- Science topic
More Chandra Sekhar Jampani's questions See All
Similar questions and discussions