How can I take subsamples of water from a larger sample in a way that allows me to measure the iron content of the subsample?

I learned how to take subsamples with a magnetic stir bar, but I noticed iron particles stick to the bar, and I am having trouble finding a standard method to solve that problem. How can I take a...

04 February 2021 4,778 1 View

Is there a paper on the development of the SQFM(AA), the Shorter questionnaire for family members affected by addiction?

I can't find anything on the validation of this tool. It measures stress/strain/coping/support for family members of substance users (or those addicted to gambling).

23 January 2021 1,463 4 View

I am looking to develop a basic oral microbiome assay. Can anyone advise on culture vessel, conditions and organisms that require minimal maintenance?

I am looking to investigate oral care products with an easy-to-prepare culture of orally derrived microorganisms.

21 January 2021 3,448 1 View

Is there any low-cost filtering system (or other tech.)to lower the salt conc. (salinity) of surface/groundwater for large scale agricultural system??

The available water (surface and groundwater) is highly saline (6-20 dS/m). It is required to produce crop (moderately saline tolerant, up to 6 dS/m). Is there any low-cost filtering system (or...

18 January 2021 5,015 3 View

What will be the life of siRNA (liquid form) at -20 degree, if kept unopened?

Hi all, I just want to know that, if we keep the siRNA in a -20 degree freezer (as mentioned in the product detail), what will be the average life of the siRNA? Can we store for more than one...

10 January 2021 6,346 6 View

Can we compare regression results (models) for different groups?

Dear Research Gate Members, I have a query regarding comparing the two groups on the basis of the regression models For Instance: I have two sector companies i.e. Public Sector and Private...

09 January 2021 9,645 8 View

PEI DNA in-vivo transfection concentration?

04 January 2021 6,908 7 View

Can I add or delete studies from systematic review during revision stage of paper publication?

I have conducted a systematic review and one of the reviewers had mentioned about missing out on some good studies from the sample. on the surface the study is on a different topic but after an...

21 December 2020 573 4 View

Is it possible to grow human breast cancer cell lines in immune competent mice?

Hello everyone, I am working in breast cancer and wanted to do in vivo studies in breast cancer mice models. Here we do not have the facility to maintain immune-deficient mice, and I have MCF-7,...

27 November 2020 1,638 3 View

What would be the recommended color/method for quad-IF?

I'm trying to perform quad-IF staining on frozen tissue sections on a fluorescent widefield (axioplan) microscope. I was wondering what people might suggest for colour/strategy for a co-IF...

13 October 2020 6,863 4 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

How to accurately measure cytokines levels for my university research proposal?

Hello, i am writing a theoretical research proposal for one of my university assignments regarding Covid-19. The brief is pretty relaxed and i have chosen to conduct a study on how the...

11 February 2021 908 5 View

Macrophages in culture not growing/ dying or detaching . Why ?

Hello, I am an undergraduate student doing my dissertation, where I isolate PBMCs from leucocyte cones in order to plate monocytes and allow for macrophage differentiation. Although the numbers...

09 February 2021 5,334 8 View

What is the best method of preparing cell supernatant samples for a Luminex assay to detect cytokines ?

I am planning to run a Luminex assay using cell supernatant to assess cytokine secretion by tumor cells. I came across different methods in literature; some suggest seeding in complete media and...

03 February 2021 4,867 3 View

What M1 and M2 markers can I use for flow cytometry ?

Hi, I'm a student and I'm doing an internship. I need to asses the M1 and M2 populations of a culture using flow cytometry. From my researches, I know that this is subject to some controversy, as...

26 January 2021 299 3 View

I am culturing murine bone marrow derived mesenchymal stromal cells, what are these cells I am observing?

Dear all, I am isolating mesenchymal stromal cells (MSCs) from the bone marrow of adult C57BL/6 mice. The culture conditions are: -IMDM + L-glut, 20% of 50:50 FBS:HS, 1% P/S -Medium replaced each...

24 January 2021 7,223 3 View