We have some Hek293T cells that have been transduced to stably express GFP. When we stimulate the cells with TNFa, we observe a significant increase in the GFP signal. Has anybody observed this before? We are using TNFa as part of a NFKB reporter assay with a GFP decrease as readout...so this GFP increase in the TNFa treated cells is a problem. To be clear, we are observing the GFP increase after TNFa stimulation even when the reporter plasmid is absent.
We thought maybe it was due to the CMV promoter driving the GFP, but we've observed a similar increase in gene expression of endogenous PABP transcripts after TNFa stimulation.
I'd love to hear any advice or thoughts as to what is going on and how we can fix it.
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