According to your process

of cryproservation if you want to do ET biotechnology process immediately you can use the ova cryopreservation.

Probably oogonia cell or somatic cell cryopreservation will be more meaningful and comparatively easy to do.

There is no simple answer. Oocyte cryopreservation has the advantage that you have a fully developed egg (or eggs) suitable after thawing for use in an IVF cycle. The disadvantages are (a) the subject has to be post pubertal, (b) the ovarian stimulation could lead to OHSS and/or ovarian torsion, and (c) it takes time to stimulate and harvest and this time might be better spent administering treatment. Tissue cryopreservation has the advantage that the procedure is fast, carries low risk and can be performed at young ages. The disadvantages are (a) re-implantaion and development of blood supply is technically difficult, and (b) there may be a risk of re-introducing cancer cells at re-implantation.

Both preservations are good, However, it depends on the purpose and Time of use...

An Oocyte is matured and developed egg... while the ovarian tissue might contain matured egg and other developing once which can also be use for other study..

For IVF Oocyte cryopreservation is better.

I agree with Thomas Kelsey (above). Ovarian Hyper Stimulation Syndrome (OHSS) has several negatives that are harmful to women, including the additional risk of causing ovarian cancer which is not medically detectable until it is well advanced.

There are studies that recommend ovarian tissue cryopreservation as the best medical option for women who need to undergo immediate cancer treatment.

Here are a few links to scientific information on this important topic:

https://www.google.com/search?q=benefits+of+ovarian+tissue+cryopreservation&oq=benefits+of+ovarian+tissue+cryopreservation+&aqs=chrome..69i57.29615j0j15&sourceid=chrome&ie=UTF-8

In my opinion, cryopreservation of whole ovary or a piece of ovary containing oocytes are more beneficial as compared to ovum. There is a big difference between oocyte (present in the ovarian follicles, germinal vesicle stage diploid in nature) and ovum (a right female gamete having first polar body with haploid genome). An ovum has very short life after ovulation (may be 48-72 hrs both in vivo as well as in vitro conditions) and cryopreservation of these female gametes could increase stress as well as fragility that may deteriorates its quality after thawing. As you know that unlike somatic cells, very few number of ovum are collected following superovulation induction protocol (ovarian stimulation) in mammals including human. Therefore, cryopreservation of oocyte within the follicular microenvironment of the ovary could be better choice and after thawing, the oocytes can be isolated the piece of ovary and processed for IVM/EVF/ET program.

Best

In my opinion the cryopreservation of oocyte is better than ovarian tissue. Several reprot it support, There is more experience with the oocytes. The ovarian cancer only has demonstrated with clomifene no with gonadotrophin.

Both cryopreservation is better but depend on your solution or cryoprotectant that used.

Medically induced "ovulation induction" using both clomifene and gonadotrophin have serious side effects for women. Here is a quote from the American Society for Reproductive Medicine (ASRM) on their website: Reproductive Facts.org:

What are some of the potential side effects of gonadotropins?

- Ovarian Hyperstimulation Syndrome (OHSS)

- Multiple Gestation (30% increase of multiple implantations)

- Ectopic (Tubal) Preganacies (increased risk of this life threatening side effect)

- Adnexal Torsion (Ovarian Twisting) which can lead to surgery and loss of ovary

- Gonadotropins and Cancer. Although early studies suggested that the risk of ovarian or other cancers of the female genital tract might be increased when exposed to medications for ovulation induction, current studies have shown no increase in any cancers with the use of fertility medications.

Unfortunately, the increased risk of ovarian cancer due to the use of "fertility drugs" is difficult to research, and a March 9, 2021 report actually states more recent research indicates that fertility drugs do increase the risk of ovarian cancer and additional studies are needed.

See: # 8 in the 10 Causes of Ovarian Cancer:

https://facty.com/conditions/cancer/10-causes-of-ovarian-cancer/8/

As I have already replied that the ovarian tissue containing follicular oocytes are better tissue for cryopreservation. And clarified a difference between oocyte and ovum. Normally oocyte is a diploid and collected only from ovary. However after ovulation, ovum is a haploid female gamete and posses first polar body.

Few basic and important aspects of oocyte biology:

Oocyte is a large specialized germ cell that is arrested at diplotene stage of meiotic cell cycle within the follicular micro-environment. These oocytes are not yet matured in the ovary and subjecting them to cryopreservation process is risky. Due to its large size (about 100 micrometer in dia) there are several possibilities of its deterioration and oocytes may become fragile due to cryopreservation that could results in the loss of its quality.

Although ovum cryopreservation is done and IVF is conducted. In spite of having a significant advances in ART till date, the IVF rate is very poor due to several limiting factors. One of the major factor is the ovum factor. Normally in Fertility clinics, ovum is collected from stimulated ovary of infertile female. In the ovarian stimulation protocol, oocytes are pushed out due to supra physiological doses of gonadotropins (FSH & LH) or by ovulation inducing drugs like Clomiphene citrate and other similar drugs. These supra physiological doses of gonadotropins and ovulation inducing drugs like Clomiphene citrate and other similar drugs may have several adverse impact of the ovum quality as stated above by Dr. Elizabeth Rex citing ASRM quote and our animal studies published in ASRM Journal Fertility Sterility :

Chaube et al., Estradiol protects clomiphene citrate–induced apoptosis in ovarian follicular cells and ovulated cumulus–oocyte complexes. (Fertil Steril
2005;84(Suppl 2):1163–72. ©2005 by American Society for Reproductive Medicine. Article Estradiol protects clomiphene citrate–induced apoptosis in o...

Clomiphene citrate inhibits gonadotropin-induced ovulation by reducing cyclic adenosine 3=,5=-cyclic monophosphate and prostaglandin E2 levels in rat ovary. Fertil Steril
2006;86(Suppl 3):1106 –11. ©2006 by American Society for Reproductive Medicine

Article Clomiphene citrate inhibits gonadotropin-induced ovulation b...

Melatonin protects against clomiphene citrate-induced generation of hydrogen

peroxide and morphological apoptotic changes in rat eggs. European Journal of Pharmacology 667 (2011) 419–424. Article Melatonin protects against clomiphene citrate-induced genera...

There is a big difference in naturally matured ovum collected after normal cycle of ovulation and ovum collected from stimulated ovary in terms of its competency. Hence to retain oocyte quality as good as naturally ovulated one, its important to take a piece of ovary containing follicular oocyte and cryopreserved. These tissues can be thawed and oocytes are collected and cultured in maturation medium so that the fist polar body is extruded (one of the most important oocyte function) in IVF Labs/Centers. After observing the first polar body, these ovum could be used for various ART programs (including IVF).