How to prevent Band/Edge Pass filter emission on Pulsed Laser excitation?

We are observing emission from both the Edge and the Bandpass filters on excitation with a Pulsed Laser. The emission is significantly high with multiple/uncontrolled peaks superimposed on the...

01 December 2020 5,449 1 View

How can i simulate loss in each layer of perovskite in Silvaco TCAD?

I am simulating a Perovskite solar cell to optimize it for best possible efficiency. I want to see losses in each layer of the perovskite solar cell, absorber, HTL and ETL and TCO . Does anybody...

15 June 2020 252 5 View

How to take the layer of glass in solar cell?

I'm willing to re-create a perovskite based solar cell but it is having glass in it and I don't know how to take glass in silvaco atlas.

06 May 2020 6,238 12 View

Is log i vs log v (scan rate) an appropriate parameter for defining the capacitive and battery behaviour.?

According to the power law i=av^b, for a material (reduced graphene oxide) to be capacitive the value of b=1 whereas b=0.5 for battery like behaviour. However, the CV shows a quasi rectangular...

26 February 2020 4,592 4 View

What is causing the delay in expression of EGFP in HEK293T transient transfection with pAAV plasmid?

I have an AAV2 backbone and I am trying to create viruses for my further experiments. I am making an AAV CMV EGFP virus as a negative control and it is giving me a problem. The EGFP expression in...

15 January 2020 4,057 1 View

Hi I am using Hofstede VSM 94 model and bit confused in calculating the different dimensions among regions??

Please provide me with a example to compute it.

16 September 2019 3,423 2 View

How to filter reads in fastq for small RNA?

Hi I am analyzing a small RNA dataset. I read in a paper that reads occurring less than 5 times were filtered. I was wondering how to it exactly. If anyone can help Thanks in advance

07 August 2018 835 2 View

Arabidopsis leaves are not bleaching in high salt concentration?

Hi there, I am doing Arabidopsis leaf disc senescence assay by using 300mM and 500mM NaCl solution. After punching leaf discs, I am keeping them in distill water (Control), and in different salt...

07 June 2018 9,061 3 View

How to do ChIP qPCR data analysis to study Histone modification?

06 February 2018 6,839 2 View

Why do bands stay on sds page gel even after performing western blot?

I have performed transfection of n and p genes of ppr virus in vero cells.. Later i went for sds page followed ny westwrn blotring... After staining my page gel i could still see some bands on my...

24 January 2018 4,202 9 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View