How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

Three-way robust ANOVA in R?

I am trying to conduct a three-way robust ANOVA in R using the WRS2 package as: t3way(Stem ~ Species*Temperature*Salinity, data = tutto) and there is an error saying : Error in if...

22 February 2021 1,674 8 View

How to improve the buffer capacity of sea water?

Hello everyone I would like to improve the buffer capacity of seawater (pH ~8) in order to dissolve as much CO2 as possible without dropping the pH below 7.5 (a range between 7 - 7.5 is...

17 February 2021 2,797 2 View

How do I dissolve cannabidiol (CBD) and crude hemp extracts in 1:1:18 ethanol:tween80:0.9% saline for IP injection in mice?

I have tried dissolving CBD and crude hemp extracts in 1:1:18 ethanol: tween80: 0.9% saline. I dissolved the drugs in ethanol first then tween, after 10 mins sonication the solutions looks ok but...

27 January 2021 6,259 3 View

Strong and extreme winds in the Arctic?

Hello everybody! Looking for a good source of information on strong and extreme winds in the Arctic (monthly values, annual extremes etc.). The form of maps (with isolines) will be most...

25 January 2021 8,984 4 View

Is there any low-cost filtering system (or other tech.)to lower the salt conc. (salinity) of surface/groundwater for large scale agricultural system??

The available water (surface and groundwater) is highly saline (6-20 dS/m). It is required to produce crop (moderately saline tolerant, up to 6 dS/m). Is there any low-cost filtering system (or...

18 January 2021 5,015 3 View

Should I add a promoter upstream of my CRISPR-inserted gene?

Hello, I want to insert a gene into my cells using sgRNA, Cas9, and an HDR template. The gene that I will insert into has its own promoter. Because I will cut the gene downstream of its start...

14 January 2021 1,170 2 View

What are the insilico tools available to recognize transcription binding sites on a promoter ?

I have to control the expression of a gene ( in vitro ). For designing an optimal promoter assay, I would like to know more about Transcription factor binding region, enhancer and repressor...

11 January 2021 7,992 3 View

Why negative controls also showing the specific bands of promotor and other regions (GUS, GFP etc.)?

Dear Researchers, I did a transformation of an empty vector (pCAMBIA1301/1302) into my passion fruit plants, for the optimization of the transformation protocol. After transformation; DNA...

06 January 2021 8,465 99 View

How can Sea water impact the hydrodynamic size of Nanoparticles differently than the same Sea water if it is autoclaved?

I am using the same sea water as autoclaved as well as non-autoclaved after adding the nanoparticle. I am getting different Hydrodynamic size for the same nanoparticle.

06 January 2021 3,184 3 View