Hi everyone, has anyone experience with specific in vitro digestion of mRNAs by RfxCas13? Could anyone let me know how specific is RfxCas13? Has anyone invesitgated collateral activity of RfxCas13 in detail? Is the crRNA really necessary to activate RfxCas13 or has this molecule general in vitro RNase activity?

Thanks for answering! Andreas

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