Andreas Brodehl

4 Questions 11 Answers 0 Followers

Questions related from Andreas Brodehl

Who can hep me to analyze WES data for runs of homozygosity?

Hello, we have performed a WES of a single human sample and I am interested to analyse the runs of homozygosity in this sample. Especially, I am interested to unterstand if segmental uniparental...

08 November 2018 7,980 1 View

What could be done to increase the chance of HDR in CRISPR-Cas9 genome editing experiments?

CRISPR-Cas9 should be used in combination with ssODN to introduce a specific point mutation...

21 July 2016 6,479 6 View

What is the chance for homology-directed repair?

Hi I want to introduce a missense mutation via CRISPR-Cas9 via HDR. I will transfect my cells with a guide-RNA and Cas9 (encoded by one plasmid) and want to cotransfect a ssODN (1 ug). I have...

12 July 2016 9,696 4 View

In vitro activity and specificity of RfxCas13 (CasRx, Cas13)

Hi everyone, has anyone experience with specific in vitro digestion of mRNAs by RfxCas13? Could anyone let me know how specific is RfxCas13? Has anyone invesitgated collateral activity of...

01 January 1970 3,832 0 View