Why I see no changes in hardness of AlCu alloy after 2 months natural aging?

08 December 2020 2,929 4 View

How to detect ethyl acetate and acetaldehyde in ethanol solution?

Hello everyone, does any one know any method to detect the existence of ethyl acetate and acetaldehyde in an ethanol medium? I have currently taken UV-VIS is there any method to detect the...

02 November 2019 1,665 3 View

Request to introduce of good resources?

25 September 2019 322 2 View

How sulfide concentration is measured by standard spectrophotometric method?

Hi dear researchers In a nature report "Amperometric inhibitive biosensor based on horseradish peroxidasenanoporous gold for sulfide determination" measuring sulfide concentration in a wastewater...

18 November 2017 8,325 3 View

Looking at the fact that miR-516b-5p along with a number microRNAs are marker of diagnosis in prefrontal cortex into Parkinson Disease

07 June 2017 956 2 View

Can we use Thioflavin T (ThT) staining for recognizing aggregated beta amyloid in lysed cell (caco2 cell line)?how can I do it?

I want to detect kinetic of beta amyloid aggregation in present of lysed cell of caco2 cell line and in present of beta amyloid? please help me

09 May 2016 9,842 3 View

Are there any structural benefits of constructing double shell domes?

I am working on Iranian Domes. There are many double-shell domes in Iran. I know that making double-shell dome helps heat insulation, but I wonder if there is any structural benefit of making...

01 February 2016 9,589 7 View

Does anyone have experience with Chimera software for RMSD calculation?

I am going to calculate RMSD between native (from PDB) and my docked complexes. I opened complexes in ArgusLab, removed their proteins, deleted all hydrogen (H) to have analogues with the same...

20 June 2015 8,875 9 View

Why when I use commercial HRP and TMB as substrate, not giving expected blue color?

Hi dear researchers We used HRP (Biobasic) in phosphate buffer (pH: 6.5) and after adding TMB, the reaction turning to light blue without adding H2O2. After adding H2O2 to reaction media, the...

01 January 1970 748 3 View

What is the difference between enzyme immobilized activity yield and recovery?

I am studying enzyme immobilized nanoparticles for catalytic applications. Research are using different names like activity yield or activity recovery. What is the difference between enzyme...

01 January 1970 2,646 12 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View