In the case of SEM (scanning electron microscopy) of Fasciola gigantica, some white spots/substances were found but what are the causes for these?

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Nicolas Barois

Hello,

a picture may be useful.

If these white spots have a geometrical form like square it is probably salt crystal. You need to wash extensively with water after fixation. You should also avoid fixation with PBS, which is highly salty.

Chandrasekaran Nagaswami

To add to the above, one can wash samples with Cacodylate buffer as rinse buffer for Electron Microscopy purposes and also use the same for fixation. This will remove salt crystal artifacts if that is the case. Hope this helps!