In the absorbance spectra, aromatic peak is decreasing with increasing salt concentration while peptide peak is increasing..what should i conclude?

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How to do protein-nanoparticle docking?

I want to do protein nanoparticle docking studies. One of my nanoparticle is 18 nm in diameter and is columnar shape, while the other one is 24 nm in diameter and spherical shaped. How can i...

07 August 2018 2,693 3 View

Fluorescence intensity of my protein is increasing after addition of nanoparticles..how can i calculate binding parameters?

I am doing protein nanoparticle interaction..addition of nanoparticles results in enhancement in fluorescence intensity..increase in concentration of nanoparticles also leads to increase in...

05 June 2018 9,150 11 View

Bone morphogenetic protein - 2 (BMP-2) is stable in which buffer? And which purification process (cation/anion exchange) should be carried out?

I tried to purify using anion exchange column (High Q), i got a peak (intensity >1500mAu). But when i did 15% SDS gel...i didn't get any band from those collected fractions. But my crude...

09 October 2016 9,839 6 View

I want to learn Molecular dynamics simulation for protein ligand interaction. How should i begin?

Sir i am currently pursuing my Ph.D. in biophysical chemistry (protein-ligand interaction). I have started doing docking (BEGINNER LEVEL) but i want to learn molecular dynamics simulation. Can you...

01 January 1970 6,840 9 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

How can we obtain a modification factor for creep parameters?

I want to obtain standard creep parameters of salt rock by creep parameters of rectangular samples. Is there a modification factor to convert creep parameters of rectangular (prismatic) rock salt...

01 March 2021 7,131 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Anyone found any research papers on solar furnace design calculations (for solar concentration)?

I am thinking about working on solar furnace with heliostats that concentrate sunlight and heat molten salt, but currently no luck with research. Not a lot of papers based on the subject. (check...

01 March 2021 5,023 5 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View

Which transition metal salt precursor is best for doping? Why?

I would like to synthesize a transition metal doped TiO2 via solgel method. Hence, I would like to know which metal salt precursor to choose among Chloride, Nitrate, and Sulphate. A clear...

27 February 2021 4,285 3 View